How should blood samples be centrifuged for proper separation of serum or plasma?

Blood samples must be centrifuged at 3500 rpm for a minimum of 10 minutes for proper separation of serum/plasma. SST/Red top tubes must be placed vertically for 30 minutes to allow blood to clot prior to centrifugation. For all blood samples collected, plasma/serum should be separated from cells within 2 hours. (Page 18)

From the official DR Lal Pathlabs DR Lal Pathlabs Lpl G Dat 005 Reference Guide manual, page 18

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